علوم زیستی دریا
Shahla Hamid; Faedeh Amini; Solmaz Shirali; Kamal Ghanemi; Ahmad Shadi
Abstract
In this study, the amount of heavy metals (nickel, cadmium, lead, copper, mercury) in brown algae Sargassum sp., Padina sp. and Cystoseira sp., sediments and the amount of mercury in water were also measured. ampling was done during winter 2016 and summer 2017 at four stations from the shores of Bushehr.Analysis ...
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In this study, the amount of heavy metals (nickel, cadmium, lead, copper, mercury) in brown algae Sargassum sp., Padina sp. and Cystoseira sp., sediments and the amount of mercury in water were also measured. ampling was done during winter 2016 and summer 2017 at four stations from the shores of Bushehr.Analysis of heavy metals in sediments and algae after chemical digestion and preparation was investigated by Savanta model atomic absorption spectrophotometer. The order obtained from the concentration of heavy metals in Padina sp. cadmium > lead > nickel > copper > mercury, for Sargassum sp. as lead > copper > nickel > cadmium > mercury and for Cystoseira sp. It is in the form of copper > lead > nickel > cadmium > mercury, among which Padina sp algae had the highest accumulation of heavy metals. The order of concentration of metals in sediments was nickel > lead > copper > cadmium > mercury. All metals, except for lead metal, have a higher average in the summer season, and the average mercury metal in water was measured at 0.23 micrograms per gram of dry weight. Correlation results showed that the concentration of heavy metals (nickel, cadmium, lead, copper, and mercury) in macroalgae Padina sp, Sargassum sp, sp. Cystoseira and their adjacent sediments in the shores of Bushehr, two algae Sargassum sp. and sp. Cystoseira have a greater ability to accumulate heavy metals.Cystoseira have a greater ability to accumulate heavy metals. According to the obtained results, the concentration of heavy metal mercury in the waters of the shores of Bushehr city with its amount in the studied algae, it seems that Sargassum sp. It is a suitable material for biological monitoring of this metal in the study area.
khadijeh nemati
Abstract
Polycyclic aromatic compounds in the marine environment are often chemically stable and resistant to the toxic effects of these compounds can be carcinogenic properties and genetic changes noted. The study of Microalgae Tetraselmis sp for the removal of aromatic compounds benzo[a]pyrene and chrysene ...
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Polycyclic aromatic compounds in the marine environment are often chemically stable and resistant to the toxic effects of these compounds can be carcinogenic properties and genetic changes noted. The study of Microalgae Tetraselmis sp for the removal of aromatic compounds benzo[a]pyrene and chrysene ring of the effluent was Bandar Imam Petrochemical . Cultivation of Microalgae in order to achieve the desired number , petrochemical wastewater samples were subjected Microalgae . The reduction in the concentration of aromatic compounds by Microalgae were measured by HPLC showed that the removal of the two compounds at a concentration of benzo[a]pyrene./5 mg L, 49/6% ± 1.8 and the concentration chrysene 5/55 mg L,33/23% ± 1.2 respectively have been . The results showed that species have the ability to reduce aromatic compounds and is also suitable for petrochemical wastewater.
علوم زیستی دریا
Razieh Nasrolahzadeh; Alireza Safahieh; Hossein Zolgharnein; Issac Zamani; Kamal Ghanemi
Abstract
Bisphenol-A (BPA) is one of the most important endocrine disrupters that has arrived to the environment, because of this reason, it has been developed into a detrimental material for human beings and other organisams. There are several ways for degradation or removal of industrial pollutants, ...
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Bisphenol-A (BPA) is one of the most important endocrine disrupters that has arrived to the environment, because of this reason, it has been developed into a detrimental material for human beings and other organisams. There are several ways for degradation or removal of industrial pollutants, in which the bacterial communities plays a major role in the biodegradation of BPA in the environment.In this study, contaminated sediments were collected in Mousa Creek. The isolates were identified by biochemical tests and 16S rRNA gene sequence analysis. The isolated Bacteri was Pseudomonas putida. Growth and degradation ability of isolated baccteri was measured in 100, 200 and 300 ppm of BPA in 24-hour intervals for 6 days. Bacterial growth rate was detected with spectrophotometer at 600 nm. Efficiency of BPA degradation was performed by Liquid-liquid extraction method and measured by HPLC. This bacteri was able to grow at concentrations of 100, 200 and 300 ppm of BPA. So, the biodegradation results from mineral salt medium (MSM) indicated that Pseudomonas putida have the best degradation efficiency at the 200 ppm of the BPA. Biodegredation result by HPLC method show that the percentage of the degradation efficiency at the 200 ppm was 82.8 percent.These results demonstrated this indigenous bacteria (Pseudomonas putida) can be used to improve the bacterial communities contaminated by Bisphenol A and to eliminate the pollutants from the costal environment.
علوم زیستی دریا
Marjan Naseri Karimvand; yadollah nikpour; Ahmad Taghavi Moghadam; Kamal Ghanemi
Abstract
Gelatin is polypeptide with high molecular weight which is derived from collagen of connective tissue, skin, bone and tendons. The most common source of gelatin in the world is skin and bone of cow and pig. Gelatin from persian Gulfs jellyfish Crambionella orsini have been successfully extracted by alkaline ...
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Gelatin is polypeptide with high molecular weight which is derived from collagen of connective tissue, skin, bone and tendons. The most common source of gelatin in the world is skin and bone of cow and pig. Gelatin from persian Gulfs jellyfish Crambionella orsini have been successfully extracted by alkaline extraction.We investigated the proximate composition, gel strength, gelling point, melting point of jellyfish gelatin. Jellyfish gelatin contained 13.1% moisture, 1.3% lipid, 2.4% ash, 78.2% protein. The gelatin showed a gel strength of 33 kPa, a gelling point of 18°C, and melting point of 23°C. The gelatin was composed of α1-chain, α2-chain, β-chain, and γ-chain Jellyfish gelatin did not show superior rheological properties to mammalian gelatin, like other fish gelatin; however, it can be used in various food and cosmetic products not requiring high gel strength.
علوم زیستی دریا
niloofar saki; yadollah nikpour ghanavaty; ahmad taghavi moghadam; kamal ghanemi
Abstract
Jellyfish is one of the poisonous animals that causing human poisoning. Found a variety of jellyfish in the Persian Gulf. Although These species cant Cause of quick death in humans But they have harmful effects on human health system and have Following are the side effects. In this study extracted Crambionella ...
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Jellyfish is one of the poisonous animals that causing human poisoning. Found a variety of jellyfish in the Persian Gulf. Although These species cant Cause of quick death in humans But they have harmful effects on human health system and have Following are the side effects. In this study extracted Crambionella Orsini Jellyfish Venom According to Bloom method and was obtained its Concentration by Biuret method and Calculated LD50 by Jung and Choi method. According to Venom concentration and its LD50 was determined that Cause of death mice 0.5 ml of venom. The use of Na-EDTA for neutralizing venom. This Chelate Was injected In two ways to mice that In both methods, Prevented death. Na-EDTA is dedicated Chelate for Calcium excretion from body that According to nuclear calciums venom is able to separated that from Venom structure and neutralize venom.
علوم زیستی دریا
M. Parviz; yadollah nikpour; A. Taghavi Moghadam; K. Ghanemi
Abstract
In this paper, the nematocyst venom of jellyfish crambionella orsini were fractionated by size-exclusion and anion-exchange chromatography. crambionella orsini is famous jellyfish in Persian gulf. The results of mentioned methods has been investigated. The crambionella orsini venoms has hemolytic activity, ...
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In this paper, the nematocyst venom of jellyfish crambionella orsini were fractionated by size-exclusion and anion-exchange chromatography. crambionella orsini is famous jellyfish in Persian gulf. The results of mentioned methods has been investigated. The crambionella orsini venoms has hemolytic activity, as the other species.After extraction of nematocyst venom, the crude venom has been partially purification by sephadex G-200 gel filtration and DEAE anion exchange chromatography. Protein elution was monitored by uv detection at 280 nm. For determine the hemolytic fraction, every fraction were injected to 3 mice via the tail vein. Finally, all data from both chromatography methods were compared. The gel filtrations first pick and the first and second pick of anion exchange chromatography showed hemolytic activity.Determination the appropriate method for purification of this venom can help to find a comprehensive method for another marine venom especially jellyfish venoms and eventually may be help to find specific antidote for jellyfish stings of these species.
علوم زیستی دریا
, A iTaherpour; bita archangi; S Ghaemmaghami; H Zolgharnein; K Ghanemi
Abstract
Brown algae of Padina sp (Adanson, 1763) are found globally across tropical coastal areas, inter-tidal and sub-tidal regions. The aim of this research was to identify morphological and molecular characteristics of padina species distributed across Lengeh Port in Persian Gulf using morphological examinations ...
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Brown algae of Padina sp (Adanson, 1763) are found globally across tropical coastal areas, inter-tidal and sub-tidal regions. The aim of this research was to identify morphological and molecular characteristics of padina species distributed across Lengeh Port in Persian Gulf using morphological examinations and rbcL chloroplast gene sequencing. For this purpose, morphological features were undertaken using valid identification keys. For molecular analysis, total genomic DNA was extracted by CTAB method with slight modifications. Amplification of fragments was carried out using rbcL primers. Analysis of genome sequences was undertaken using genetic softwares of Chromas, BioEdit and MEGA6 and phylogenetic trees were constructed by two models of Neighbor Joining (NJ) and Maximum Likelihood (ML). Results indicated that two identical species of padina (P. boergesenii and P. australis) in this research shared common cluster with 99% probability and showed high genetic similarities among related compared padina species submitted in NCBI Genbank.
علوم زیستی دریا
niloofar saki; yadollah nikpour ghanavaty; ahmad taghavi moghadam; kamal ghanemi
Abstract
The aim of this study is evaluate the effect of temperature changes to reduce toxicity, jellyfish venom Crambionella orsini. Venom extraction was done according to Bloom method. To breaking wall of nematocysts capsule, at first, sonication it and than The resulting solution was centrifuged. To evaluate ...
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The aim of this study is evaluate the effect of temperature changes to reduce toxicity, jellyfish venom Crambionella orsini. Venom extraction was done according to Bloom method. To breaking wall of nematocysts capsule, at first, sonication it and than The resulting solution was centrifuged. To evaluate the effect of temperature on the venom, it is heated at different temperatures and then injected into SoRI mice. After catching jellyfish Crambionella orsini from Arvand nidstream estuary umbrellas edge and tentacles jellyfish were separated and put were in water that was removed on the same area. Toxins LD50 were calculated by Jung and Choi method .and using statistical analysis to obtain minimal lethal dose of poison done by the 2007Excel. The results showed that the venome of jellyfish Crambionella orsini, like venome of other animals is, based on a protein and that is sensitive to heat. This venome at a temperature of 48 °C is disabled and lose their structure and also its minimum lethal dose is 0.5 ml.
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Volume 11, Issue 4 , March 2013, , Pages 46-56
علوم زیستی دریا
Masoomeh Mahmoodi; Alireza Safahieh; Yadollah Nikpour; Kamal Ghanemi
Volume 11, Issue 1 , November 2012, , Pages 58-67
Abstract
In order to study concentration of polycyclic aromatic hydrocarbons in seawater from Bushehr coast and for comparison with available guidelines samples of seawater were collected from five different stations along the Bushehr coast in August and February 2011. PAHs were extracted by Hexane solvent and ...
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In order to study concentration of polycyclic aromatic hydrocarbons in seawater from Bushehr coast and for comparison with available guidelines samples of seawater were collected from five different stations along the Bushehr coast in August and February 2011. PAHs were extracted by Hexane solvent and analyzed using HPLC system (Knauer). Results showed that tPAHs concentration in seawater were 31.0, 20.8, 4.0, 17.6 and 12.3 µg l-1, in August and 38.4, 23.0, 5.4, 19.3 and 17.2 µg l-1 in February respectively, at stations Rafael, Sheghab, Abshirinkon, Lian and Helyleh. The concentrations of tPAHs in the seawater were not significantly different during August and February (P>0.05). Significant difference was observed between tPAHs concentration between the stations (P<0.05). The tPAHs concentration was maximum in Rafael and its minimum was found in Abshirinkon., The tPAHs concentration in Bushehr area was relatively higher compared to other locations of the world. Even though concentrations of anthracene, phenanthrene, fluoranthene and pyrene were above the Canadian Environment Guidance, the carcinogenic compounds appeared in lower concentrations than the non-carcinogenic PAHs. Since Bushehr coastal waters is contaminated by PAHs, precise monitoring and control of oil discharge into the coastal waters as well as reduction of urban effluents input should be undertaken. Meanwhile the continuous monitoring of PAHs compounds in the area is recommended.